Nuclear factor κB represses the expression of latent membrane protein 1 in Epstein-Barr virus transformed cells

Figure 2 Overexpression of nuclear factor-κB decreases the expression of latent membrane protein 1 in Epstein-Barr virus-transformed cells. IB4 cells were transfected with CD4 expression plasmid along with pcDNA3 (vector) or nuclear factor κB (NF-κB) expression plasmids (p50 and p65 expression plasmids at 1:1 ratio). One day after the transfection, the transfected cells were enriched using the CD4 magnetic beads, and the cell lysates were used for Western blot analysis. The identity of the proteins is as shown. The right panel: IB4 cells were transfected with the indicated plasmid as shown at the top along with NF-κB specific reporter construct and Renilla luciferase reporter plasmid. One day later, luciferase and Renilla luciferase activities were measured. Relative promoter reporter activities are shown. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; LMP1: Latent membrane protein-1.