Inhibition of the microglial voltage-gated proton channel 1 channel ameliorates diabetes-associated cognitive dysfunction by regulating axon demyelination

Figure 7 High glucose affects M1-type microglia by activating glucose-regulated protein 78 in microglia. The levels of ferroptosis markers, including ferritin heavy chain, ferritin light-chain, CCAAT/enhancer-binding protein homologous protein, and glucose-regulated protein 78, were significantly increased in high glucose cultured microglia, which were restored in the voltage-gated proton channel 1 (Hv1) si-RNA-treated cells. In addition, when the YUM70 was added, the effect of siRNA-Hv1 was enhanced, while the control RNA did not affect the expression of these markers. ^dP < 0.05. Hv1: The voltage-gated proton channel 1; FTH: Ferritin heavy chain; FTL: Ferritin light-chain; GRP78: Glucose-regulated protein 78; CHOP: CCAAT/enhancer-binding protein; HG: High glucose; PBS: Phosphate buffer saline.