Inhibition of the microglial voltage-gated proton channel 1 channel ameliorates diabetes-associated cognitive dysfunction by regulating axon demyelination

Figure 6 The voltage-gated proton channel 1 gene is involved in the high glucose and high osmolarity-induced production of interleukin-1β and tumor necrosis factor-α. The interleukin (IL)-1β levels decreased in microglial cells after adding siRNA-the voltage-gated proton channel 1 but remained the same after treatment with control RNA. This finding aligns with the observed alterations in IL-1β levels in the animal corpus callosum, where the expression of IL-1β was unaffected by using control RNA. ^dP < 0.05. The scale bar = 50 μm. Hv1: The voltage-gated proton channel 1; Iba-1: Ionized calcium-binding adaptor molecule 1; HG: High glucose; PBS: Phosphate buffer saline; IL: Interleukin.