Tanshinone IIA improves Alzheimer’s disease via RNA nuclear-enriched abundant transcript 1/microRNA-291a-3p/member RAS oncogene family Rab22a axis

Figure 1 The impact of tanshinone IIA in a murine model of Alzheimer’s disease, with the long non-coding RNA nuclear-enriched abundant transcript 1 as a potential target. A: Chemical structure of tanshinone IIA (Tan-IIA); B: Hematoxylin and eosin staining of brain tissues from control, Alzheimer’s disease, and Tan-IIA-treated mice (400 × magnification); C: Results of the 2’,7’-dichlorofluorescin diacetate analysis of the reactive oxygen species ratio in mouse brain tissues, either with or without prior Tan-IIA treatment; D-J: Enzyme-linked immunosorbent assay analysis of oxidative stress factors (malondialdehyde; nitric oxide; superoxide dismutase; glutathione) and neuroinflammation markers (tumor necrosis factor-alpha; interleukin 1β; interleukin 6) in mouse brain tissues; K: GEO2R web tool analysis of the GSE150696 data set for long non-coding RNA screening; L: Analysis the expression of nuclear-enriched abundant transcript 1 in Tan-IIA-treated mouse brain tissues. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. AD: Alzheimer’s disease; Tan-IIA: Tanshinone IIA; ROS: Reactive oxygen species; MDA: Malondialdehyde; NO: Nitric oxide; SOD: Superoxide dismutase; GSH: Glutathione; TNF-α: Tumor necrosis factor-alpha; IL-1β: Interleukin 1β; IL-6: Interleukin 6.