Tanshinone IIA improves Alzheimer’s disease via RNA nuclear-enriched abundant transcript 1/microRNA-291a-3p/member RAS oncogene family Rab22a axis

Table 2 Primer sequences used for reverse transcription quantitative polymerase chain reaction analysis

Primers	Forward primer 5’-3’	Reverse primer 5’-3’
NEAT1	TGGAGATTGAAGGCGCAAGT	AAGCACGGAACCTAGGCAAA
miR-291a-3p	ACACTCCAGCTGGGAAAGTGCTTCCACTTT	CTCAACTGGTGTCGTGGA
U6	CTCGCTTCGGCAGCACA	AACGCTTCACGAATTTGCGT
Rab22a	ATCAATCCAACCATAGGGGCAT	TTGGTGCCAATGCACGAAATC
GAPDH	GTGGCAAAGTGGAGATTGTTGCC	GATGATGACCCGTTTGGCTCC

U6 was used to normalize miR-291a-3p expression, and GAPDH was used to normalize nuclear-enriched abundant transcript 1 mRNA expression. NEAT1: Nuclear-enriched abundant transcript 1.