KAT7/HMGN1 signaling epigenetically induces tyrosine phosphorylation-regulated kinase 1A expression to ameliorate insulin resistance in Alzheimer’s disease

Figure 5 KAT7 epigenetically induces dual-specificity tyrosine phosphorylation-regulated kinase-1A expression. A: RNA level of KAT7 in neurons after transfection of siKAT7-1, siKAT7-2, or siKAT7-3 was measured using qPCR; B: RNA level of dual-specificity tyrosine phosphorylation-regulated kinase-1A (DYRK1A) in neurons after siKAT7-3 transfection was measured using qPCR; C: Chromatin immunoprecipitation assay to measure enrichment of H3K14ac on DYRK1A gene; D: RNA level of HMGN1 in neurons after transfection of siHMGN1-1, siHMGN1-2, or siHMGN1-3 was measured using qPCR; E: RNA level of DYRK1A in neurons after transfection of siHMGN1-3 was measured using qPCR; F and G: RNA and protein levels of DYRK1A in neurons after siKAT7-3 transfection with or without KAT7 overexpression vectors was measured using qPCR. ^bP < 0.01. DYRK1A: Dual-specificity tyrosine phosphorylation-regulated kinase-1A.