Exosomal miR-320e through wnt2targeted inhibition of the Wnt/β-catenin pathway allevisate cerebral small vessel disease and cognitive impairment

Figure 4 miR-320e inhibited the Wnt/β-catenin pathway through targeting Wnt2. A and B: Cell transfection images were shown; C-E: The Western-Blot results showed that the expression of Wnt2, FZD2, Axin2, GSK3β, and β-catenin in the miR-320e mimics group was inhibited compared to that in the control group, while this inhibition was effectively blocked by Wnt2 silencing compared to the siRNA control; F and G: The results of real-time PCR showed similar trends to the protein levels of Wnt2, FZD2, Axin2, GSK3β, and β-catenin with overexpressed miR-320e and knockdown of Wnt2. PCR: Polymerase chain reaction. ^aP < 0.05.