Exosomal miR-320e through wnt2targeted inhibition of the Wnt/β-catenin pathway allevisate cerebral small vessel disease and cognitive impairment

Figure 3 Representative protein blots of Wnt2, FZD2, Axin2, GSK3β, and β-catenin expression under hydrogen peroxide. A and B: The Western-Blot results showed that Wnt2 expression in the hydrogen peroxide group increased compared to that in the control group; C: The PCR results showed that Wnt2 expression in the hydrogen peroxide group increased compared to that in the control group; D-F: Expression of Wnt2, FZD2, Axin2, GSK3β, and β-catenin expression after treating with WIF1 and R-Spondin under hydrogen peroxide were similar and further enhanced, respectively, compared to that in the groups without hydrogen peroxide; G and H: The PCR results showed that GSK3β in the WIF + H₂O₂ group increased, and the other mRNA expression levels remained similar. PCR: Polymerase chain reaction. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001.