Exosomal miR-320e through wnt2targeted inhibition of the Wnt/β-catenin pathway allevisate cerebral small vessel disease and cognitive impairment

Figure 2 miR-320e could bind to Wnt2 to inhibit the Wnt/β-catenin pathway. A: The results of the dual-luciferase reporter gene experiment; B: Binding sequence between Wnt2 mRNA and miR-320e; C and D: Protein blots were listed in the column. After cotransfection of the miR-320e mimic, western blotting showed that Wnt2, FZD2, Axin2, GSK3β, and β-catenin expression increased compared with that of the control group. After cotransfection of the miR-320e inhibitor, western blotting showed that Wnt2, FZD2, Axin2, GSK3β, and β-catenin expression decreased compared with that of the control group; E: Cell image after cotransfection of cells. The image above showed transfection with miR-320e mimic and the one below showed transfection with miR-320e inhibitor. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001.