Acupuncture at Back-Shu point improves insomnia by reducing inflammation and inhibiting the ERK/NF-κB signaling pathway

Figure 5 Acupuncture at Back-Shu point inhibited the ERK/NF-κB signaling pathway in rats. A: The expression of ERK/NF-κB pathway-related proteins in rat hippocampus was detected by western blot; B: The relative protein expression level of RAF-1, MEK-2, ERK1/2 and NF-κB in rat hippocampus; C: The expression levels of ERK/NF-κB pathway-related proteins in the hippocampus in rats were detected by immunohistochemistry, scale bars = 100 μm (× 200); D: The gray values of RAF-1, MEK-2, ERK1/2 and NF-κB. ^aP < 0.05, compared to the Con group; ^bP < 0.05, compared to the Model group.