Magnesium-L-threonate exhibited a neuroprotective effect against oxidative stress damage in HT22 cells and Alzheimer’s disease mouse model

Figure 2 Magnesium-L-threonate treatment suppressed the elevated reactive oxygen species level and hypoxia-inducible factor-1α protein expression in the amyloid β_25-35-exposed HT22 cells. A, B: The percentages of dichloro-dihydro-fluorescein diacetate positive cells of each group; C: Protein band images of hypoxia-inducible factor (HIF)-1α and glyceraldehyde-3-phosphate dehydrogenase of each group; D: The HIF-1α protein expression level of each group. n = 3. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs former group. Aβ: Amyloid β; MgT: Magnesium-L-threonate; HIF: hypoxia-inducible factor; DCFH-DA: dichloro-dihydro-fluorescein diacetate; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.