Selective ablation of type 3 adenylyl cyclase in somatostatin-positive interneurons produces anxiety- and depression-like behaviors in mice

Figure 5 Parvalbumin-Cre recombinase:type 3 adenylyl cyclase^f/f mice did not show anxiety-like behavior. A: Strategy for generation of parvalbumin-Cre recombinase:type 3 adenylyl cyclase^f/f (PV-Cre:AC3^f/f) mice in which AC3 was selectively deleted in PV⁺ interneurons; B: Double immunohistochemistry showing the representative micrographs of AC3 protein expression (red) in PV⁺ interneurons (green) from AC3^flox/flox control mice (left) and PV-Cre:AC3^f/f mice (right). Solid arrows indicated that PV⁺ interneurons have well colocalization with AC3 in AC3^flox/flox control mice. Empty arrow indicates that AC3 immunostaining disappeared in PV⁺ interneurons in PV-Cre:AC3^f/f mice. Scale bar: 20 μm; C-E: Showing PV-Cre:AC3^f/f mice showed similar total distance (C), percentage of center distance (D) and center time (E) in the open field test to control group; F-H: PV-Cre: AC3^f/f mice exhibited comparable total arm entries (F), open arm time (G) and percentage of open arm entries (H) in elevated plus maze test compared with AC3^flox/flox mice and PV-Cre mice. Bars represent the mean ± standard error of the mean. Data were analyzed using one-way analysis of variance followed by post hoc Tukey's multiple comparisons test. n = 8 for AC3^flox/flox group, n = 8 for PV-Cre group, n = 12 for PV-Cre:AC3^f/f group.