Selective ablation of type 3 adenylyl cyclase in somatostatin-positive interneurons produces anxiety- and depression-like behaviors in mice

Figure 3 Somatostatin-positive-Cre recombinase:type 3 adenylyl cyclase^f/f mice showed anxiety-like behavior. A: Strategy for generation of somatostatin-positive-Cre recombinase: type 3 adenylyl cyclase^f/f (SST-Cre:AC3^f/f) mice in which type 3 adenylyl cyclase (AC3, ADCY3) was selectively ablated in SST⁺ interneurons; B: Fluorescence in situ hybridization showing the representative micrographs of ADCY3 mRNA expression (red) in SST⁺ interneurons (green) from AC3^flox/flox control mice (left) and SST-Cre:AC3^f/f mice (right). Solid arrows indicated that SST⁺ interneurons are co-expressed ADCY3 mRNA in AC3^flox/flox control mice. Empty arrow indicated that ADCY3 mRNA fluorescence is missing in SST⁺ interneurons in SST-Cre:AC3^f/f mice. Scale bar: 10 μm; C-E: Showing SST-Cre:AC3^f/f mice showed less total distance (C) but comparable percentage of center distance (D) and center time (E) in the open field test; F-H: SST-Cre:AC3^f/f mice exhibited decreased total arm entries (F), open arm time (G) and percentage of open arm entries (H) in the elevated plus maze test. Bars represent the mean ± standard error of the mean. Data were analyzed using one-way analysis of variance followed by post hoc Tukey’s multiple comparisons test. n = 12 for AC3^flox/flox group, n = 8 for SST-Cre group, n = 15 for SST-Cre:AC3^f/f group. ^aP < 0.05 vs AC3^flox/flox. ^bP < 0.01 vs AC3^flox/flox. ^cP < 0.01 vs SST-Cre. ^dP < 0.05 vs SST-Cre. ^eP < 0.001 vs SST-Cre.