Selective ablation of type 3 adenylyl cyclase in somatostatin-positive interneurons produces anxiety- and depression-like behaviors in mice

Figure 1 Expression of type 3 adenylyl cyclase in cortical somatostatin-positive and parvalbumin-positive GABAergic interneurons. A and B: Representative images showing type 3 adenylyl cyclase (AC3) expression in somatostatin-positive (SST⁺) interneurons at low (A) and high (B) magnifications in the prefrontal cortex brain slice. Solid arrows in B indicate SST⁺ interneurons, which are also AC3-positve (SST-AC3⁺ cells). Empty arrow in B indicates SST⁺ interneurons, which are AC3-negative (SST-AC3^- cells); C: Proportion of AC3 expressed in cortical SST⁺ neurons, n = 6; D and E: Representative images showing AC3 expression in parvalbumin-positive (PV⁺) interneurons at low (D) and high (E) magnifications in the prefrontal cortex brain slice. Solid arrows indicate PV⁺ interneurons, which are also AC3-positve (PV-AC3⁺ cells). Empty arrow in E indicates PV⁺ interneurons, which are AC3-negative (PV-AC3^- cells); F: Proportion of AC3 expressing in cortical PV⁺ neurons, n = 5. Scale bars represent 100 μm in D and 20 μm in E, respectively.