BDNF methylation and mRNA expression in brain and blood of completed suicides in Slovenia

Table 1 Comparisons of the methylation levels across the BDNF regions in the control subjects and suicide victims

Tissue	Study group	BDNF region (% methylated)
		I1	I2	II	IV	VI
Brain-BA9	C	4.55 ± 0.45	4.43 ± 0.39	8.48 ± 0.91	3.81 ± 0.32	3.37 ± 0.30
	SV	4.15 ± 0.56	4.28 ± 0.32	8.78 ± 1.09	4.03 ± 0.46	3.05 ± 0.23
	P value	0.260	0.557	0.667	0.418	0.090
Brain-Hippocampus	C	4.76 ± 0.48	4.16 ± 0.44	10.19 ± 0.51	3.74 ± 0.46	3.46 ± 0.34
	SV	4.40 ± 0.39	4.60 ± 0.43	10.30 ± 1.10	3.66 ± 0.37	3.52 ± 0.31
	P value	0.222	0.147	0.851	0.772	0.774
Blood	C	7.81 ± 1.03	6.83 ± 0.64	15.95 ± 1.36	5.71 ± 0.39	5.38 ± 0.95
	SV	7.00 ± 0.80	5.67 ± 0.57	14.26 ± 1.2	5.54 ± 0.38	4.80 ± 0.46
	P value	0.198	0.007a	0.067	0.521	0.245

^aPcorr < 0.05 (Benjamini–Hochberg method). The studied regions are labelled with Roman numerals (I, II, IV, and VI) according to the vicinity of the exons 1, 2, 4, or 6. The region preceding the first exon (I) is divided into two parts, I1 and I2 due to technical reason of maximum amplicon length recommendation for 454 GS Junior sequencing system (400 bp/region, including 454 GS Junior sequencing primers). Data are means ± 95% confidence interval. C: Controls; SV: Suicide victims. BA9: Brodmann area 9.