Comparison of three administration modes for establishing a zebrafish seizure model induced by N-Methyl-D-aspartic acid

Figure 5 Comparison of the three administration routes on seizure score and onset time. Seizure activity scores and onset times following intravitreal injection, intraperitoneal injection and immersion with 0.5 mol/L, 16 mg/kg and 500 μmol/L N-Methyl-D-aspartic acid (NMDA), respectively. A: The mean seizure scores (± SE) for each route plotted against time after NMDA treatment; B: The latency of seizure onset after NMDA treatment. Bars represent mean ± SE of the time lag to the onset of the first convulsion using each method. Data were analyzed using one-way ANOVA and t-test for the different routes (n = 6) (^aP < 0.05). NMDA: N-Methyl-D-aspartic acid; I.V.: Intravitreal injection; I.P.: Intraperitoneal injection; I.M.: Immersion.