Copyright
©The Author(s) 2016.
World J Exp Med. May 20, 2016; 6(2): 37-54
Published online May 20, 2016. doi: 10.5493/wjem.v6.i2.37
Published online May 20, 2016. doi: 10.5493/wjem.v6.i2.37
Figure 2 Adeno-associated virus vector-based microRNA targeting strategies to improve cardiac - specific gene therapy.
Cardiac-specific gene transfer of AAV vectors can be obtained by different targeting strategies, including transcriptional targeting using cardiac hybrid promoters[68,122], transductional targeting[109-113,187,188] or microRNA-regulated post-transcriptional transgene suppression. miR-OFF system (right site): Expression of miR-TS-bearing transgenes can be selectively suppressed in non-cardiac tissue by microRNAs, which are expressed in these tissues, but not in the heart. For the miR-OFF system (left site): If microRNAs are selectively down-regulated in cardiovascular diseases, transgene expression may be restricted to these cells by use of miR-TS corresponding to down-regulated microRNAs. If microRNAs are up-regulated in cardiovascular diseases, corresponding miR-TS can be used in the miR-ON system[178] for regulation of a repressor protein to restrict transgene expression to respective cells. Shown microRNAs are specifically expressed in mouse tissues (unpublished). AAV: Adeno-associated virus; pA: Polyadenylation site; CMV: Cytomegalovirus; MLC: Myosin light chain; MHC: Myosin heavy chain; TNT: Troponin T.
- Citation: Geisler A, Fechner H. MicroRNA-regulated viral vectors for gene therapy. World J Exp Med 2016; 6(2): 37-54
- URL: https://www.wjgnet.com/2220-315X/full/v6/i2/37.htm
- DOI: https://dx.doi.org/10.5493/wjem.v6.i2.37