Changing insights in the diagnosis and classification of autosomal recessive and dominant von Willebrand diseases 1980-2015

Table 3 Desmopressin challenge test (0.3 μg/kg in 100 mL physiological saline intravenously over 30 min) proposed by the International Society on Thrombosis and Haemostasis

Blood sampleDDAVP	At 15 min	After DDAVP				After DDAVP12 h
		1 h	2 h	4 h	6 h
Ivy BT	+	-	+	-	-	+
PFA-100	+	+	+	+	+	+
RIPA	+	+	+	+	+	+
FVIII:C	+	+	+	+	+	+
VWF:Ag	+	+	+	+	+	+
VWF:RCo	+	+	+	+	+	+
VWF:CB	+	+	+	+	+	+
VWF:MM	+	+	+	+	+	+
VWF propeptide	+	+	+	+	+	+

This challenge test has been used at the Goodheart Institute, Rotterdam since 1992 to calculate the recovery and half life times of FVIII:C and VWF parameters for the diagnosis and charaterization of VWD type 1, 2 and 3[18]. VWD: Von Willebrand disease; VWF: Von Willebrand factor; RIPA: Ristocetin induced platelet aggregation; DDAVP: Desmopressin; MM: Multimers.