Tbl3 encodes a WD40 nucleolar protein with regulatory roles in ribosome biogenesis

Figure 7 Knockdown of transducin β-like 3 inhibits cellular proliferation. A: Growth curves of stable MPRO/pMKO (negative control) vs MPRO/pMKO-transducin β-like 3 (Tbl3) small hairpin RNAs (shRNAs) transfectants. Each culture was started with 105 low-passage stable transfectants. Each data symbol represents the mean of triplicates and the standard deviation; B: Tbl3 knockdown markedly inhibits the proliferation of fibroblasts, left: LAP3/Neg; right: LAP3/Tbl3 shRNA. LAP-3 fibroblasts were transfected with pMKO or pMKO-Tbl3 shRNA and selected with puromycin (1.5 μg/mL) for 5-10 d to allow colony formation of stable transfectants. Colonies were fixed and stained with Coomassie blue in situ and photographed. Plates shown are representative of three independent experiments.