Tbl3 encodes a WD40 nucleolar protein with regulatory roles in ribosome biogenesis

Figure 6 Knockdown of transducin β-like 3 has no discernable effect on ribosome profiles. Cell lysates prepared from equal numbers of MPRO/pMKO vs pMKO-transducin β-like 3 (Tbl3) small hairpin RNAs (shRNAs) stable transfectants were analyzed by sucrose gradient centrifugation in the absence of cycloheximide, followed by absorbance measurement at 254 nmol/L. (The omission of cycloheximide allowed polysomes to dissociate into 80S monosomes to facilitate the comparison of the total amount of 80S monosomes.) The peaks corresponding to the 40S and 60S ribosomal subunits and the 80S monosomes are indicated. The slight difference in the 80S monosome peaks is due to unequal loading and well within experimental variations.