Nanoparticle-linked antagonist for leptin signaling inhibition in breast cancer

Figure 7 Determination of the effect of IONP-LPrA2 on survival of breast cancer cells treated with chemotherapeutics. MDA-MB-231, HCC1806, and MCF-7 cells were treated with an effective dose of the chemotherapeutics. A: Cisplatin (Cis); B: Cyclophosphamide (CTX); C: Doxorubicin (Dox); D: Paclitaxel (PTX) plus IONP-LPrA2 (0.0036 pmol/L). The cells were seeded in 6 well plates and treated with chemotherapeutics at effective concentrations determined in Figure 6 for 1-6 d. The treatment concentrations were MDA-MB-231 (Cis 0.001 μmol/L, CTX 0.5 μmol/L, Dox 0.4 μmol/L, PTX 0.5 μmol/L); HCC1806 (Cis 0.036 μmol/L, CTX 1 μmol/L, Dox 10 μmol/L, PTX 0.5 μmol/L); and MCF-7 (Cis 0.036 μmol/L, CTX 5 μmol/L, Dox 0.01 μmol/L, PTX 1 μmol/L). Percent of survival was determined by the Annexin V/FITC and PI assay. The relative survival was determined by multiplying the percentage of live cells by the total cell count. Percent viability was significantly decreased in cells treated with chemotherapeutic compared to basal (untreated) cells, ^aP < 0.05. Cells treated with chemotherapeutic and IONP-LPrA2 differed significantly from those treated with chemotherapeutic alone, ^cP < 0.05.