Orally administered extract from Prunella vulgaris attenuates spontaneous colitis in mdr1a-/- mice

Figure 4 Evaluation of T cell and B cell subsets in the cecal tonsil of mdr1a^-/- mice treated with prunella vulgaris ethanolic extract. Cecal tonsils were excised at necropsy, single cell suspensions prepared and labeled for flow cytometric analysis as described in Materials and Methods. Absolute numbers of (A) CD4+ T cells; (B) CD8ß+ T cells; and (C) B220+PNA+ germinal center B cells in the cecal tonsils of mice. ^aP < 0.05, ^bP < 0.01 compared to mdr1a^-/- vehicle as calculated by Kruskal-Wallis test. ^cP < 0.05 compared to mdr1a^-/- vehicle as calculated by unpaired t-test. The n for each group is equal to that noted in Figure 1 and data are representative of two independent experiments. Vehicle-treated FVB^WT mice n = 5-6, vehicle-treated mdr1a^-/- mice n = 8-10, P. vulgaris-treated mdr1a^-/- mice n = 5-7.