Evaluation of DNA synthesis with carbon-11-labeled 4′-thiothymidine

Figure 5 Time courses of 4’-[methyl-¹¹C]-thiothymidine activity in the mouse duodenum. The data is presented as the SUVmean in the indicated fractions (n = 3-5). Rapid DNA separation was performed using the DNAzol reagent[30]. The increase in total tissue radioactivity paralleled that of DNA incorporated radioactivity. The soluble fraction (4DST itself, phosphorylated 4DST, and other metabolites) was low and stable at 30 min after tracer injection. The contribution of the DNA incorporation process overshadows both the phosphorylation and dephosphorylation rates at later phases of the PET scan. PET: Positron emission tomography; SUV: Standardized uptake value.