Copyright
©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Cardiol. Apr 26, 2017; 9(4): 320-331
Published online Apr 26, 2017. doi: 10.4330/wjc.v9.i4.320
Published online Apr 26, 2017. doi: 10.4330/wjc.v9.i4.320
Dissection of Z-disc myopalladin gene network involved in the development of restrictive cardiomyopathy using system genetics approach
Qingqing Gu, Biao Xu, Department of Cardiology, Drum Tower Clinic Hospital, Nanjing Medical University, Nanjing 211166, Jiangsu Province, China
Qingqing Gu, Lu Lu, Department of Genetics, Genomics and Informatics, University of Tennessee Health Science Center, Memphis, TN 38103, United States
Uzmee Mendsaikhan, the Heart Institute, Cincinnati Children’s Hospital Medical Center and Mongolian National University of Medical Sciences, Ulaanbaatar 14210, Mongolia
Zaza Khuchua, the Heart Institute, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH 45229, United States
Byron C Jones, the Neuroscience Institute, Department of Genetics, Genomics and Informatics, University of Tennessee Health Science Center, Memphis, TN 38103, United States
Jeffrey A Towbin, Enkhsaikhan Purevjav, Department of Pediatrics, the Heart Institute, University of Tennessee Health Science Center, Memphis, TN 38103, United States
Jeffrey A Towbin, Enkhsaikhan Purevjav, Children’s Foundation Research Institute, Le Bonheur Children’s Hospital Memphis, TN 38103, United States
Jeffrey A Towbin, Pediatric Cardiology, St. Jude Children’s Research Hospital, Memphis, TN 38103, United States
Author contributions: Gu Q performed experiment and data analysis; Mendsaikhan U maintained animals, performed the in vivo experiments and proteomics analysis; Khuchua Z assisted in performing proteomics analysis; Xu B supported post-doc; Jones BC edited manuscript; Lu L performed data analysis and drafted manuscript; Towbin JA supervised all experimentation, edited and revised the manuscript; Xu B supported post-doc, designed experiment; Purevjav E acquired conception and design of the study, performed proteomics analysis and interpretation, drafted and edited the manuscript.
Supported by National Institutes of Health, Nos. R01 HL128350 (LL), R01 HL53392 and R01 HL087000 (JAT).
Institutional animal care and use committee statement: All animal studies were approved by institutional IACUC of the University of Tennessee Health Science Center (UTHSC).
Conflict-of-interest statement: To the best of our knowledge, no conflict of interest exists.
Data sharing statement: Resources: Principles and Guidelines for Recipients of NIH Grants and Contracts” issued in December, 1999. Dr. Lu Lu is responsible for coordinating data sharing through GeneNetwork (GN) at the: http://www.genenetwork.org/webqtl/main.py. GN is a group of linked data sets and tools used to study complex networks of genes, molecules, and higher order gene function and phenotypes. GN combines more than 25 years of legacy data generated by hundreds of scientists together with sequence data (SNPs) and massive transcriptome data sets (expression genetic or quantitative trait locus data sets). GN connected to numerous links to the UCSC and Ensembl Genome Browsers, PubMed, Entrez Gene, GNF Expression Atlas, ABI Panther, and WebGestalt provide users with rapid interpretive information about genomic regions, published phenotypes and genes.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Enkhsaikhan Purevjav, MD, PhD, Department of Pediatrics, the Heart Institute, University of Tennessee Health Science Center, 71 S Manassas Street, Memphis, TN 38103, United States. epurevja@uthsc.edu
Telephone: +1-901-4481117 Fax: +1-901-2864551
Received: December 6, 2016
Peer-review started: December 7, 2016
First decision: January 16, 2017
Revised: February 9, 2017
Accepted: February 28, 2017
Article in press: March 2, 2017
Published online: April 26, 2017
Processing time: 143 Days and 2.2 Hours
Peer-review started: December 7, 2016
First decision: January 16, 2017
Revised: February 9, 2017
Accepted: February 28, 2017
Article in press: March 2, 2017
Published online: April 26, 2017
Processing time: 143 Days and 2.2 Hours
Core Tip
Core tip: Myopalladin (Mypn) is one of genes associated with many types of familial cardiomyopathies including dilated, hypertrophic and restrictive cardiomyopathy (RCM). Using systematic genetics approach, we constructed Mypn co-expression networks of similarly regulated genes that function within defined biological processes. Several novel Mypn-interacting genes with potential important role in the development of RCM were discovered.