Identification of host miRNAs that may limit human rhinovirus replication

Figure 3 Prolonged DICER knock-down leads to higher levels of viral RNA. BEAS-2B cells were transfected for three rounds (using 30 nmol/L of siRNA) and then infected with human rhinovirus (HRV)-1B at MOI 0.01. Cells collected just before infection were used to measure DICER mRNA levels (A) and miR-30a-5p expression (B) by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). HRV-1B RNA (C) and IFNB1 mRNA (D) levels in infected cells were measured by RT-qPCR at the indicated time points. In (D) the time point “-1” refers to uninfected cells, collected just before infection. In (C) all the samples were normalised as done for data in Figure 2 [0 h post-infection (HPI), MOI 0.001 sample as calibrator]. Plotted values represent the mean ± SD, of three independent experiments. For all panels, unpaired t test was used to calculate the P values for anti-DICER vs negative control siRNA samples.^aP < 0.05; ^bP < 0.01. NS: Not significant; MOI: Multiplicity of infection.