Thioredoxin and glutaredoxin-mediated redox regulation of ribonucleotide reductase

Figure 4 Activity profile of mouse ribonucleotide reductase in the presence of the thioredoxin and glutaredoxins system. Mouse R1 (120 μg/mL) and R2 (40 μg/mL) were assayed with dithiothreitol, thioredoxin (Trx) and glutaredoxin (Grx) systems. The Trx system contained 3.6 μmol/L Trx1, NADPH and TrxR. The Grx system contained 1 μmol/L Grx1, 4 mmol/L glutathione , NADPH and glutathione reductase. Combinations of 3.6 μmol/L Trx1 or the whole Trx system with the Grx system were also monitored (data adapted from Zahedi Avval et al[26]).