Effect of alcohol exposure on hepatic superoxide generation and hepcidin expression

Figure 1 Genotyping and expression. A: Genotyping of mice. Genomic DNA, isolated from the tails of mice by using a commercial kit (Promega), was employed to amplify wild type and mutant Sod2 allele by PCR, as described[38]. Heterozygous (Sod2^+/-) mice were expressing both mutant (197 bp) and wildtype (457 bp) Sod2 alleles whereas littermate control (LMC) mice were expressing only wildtype (457 bp) Sod2 allele, as shown by the agarose gel stained by ethidium bromide; B: Hepatic Sod1 and Sod2 mRNA expression in Sod2^+/- and LMC mice were determined by RT-PCR, as described in Methods. Gapdh was used as the endogenous control gene. PCR: Polymerase chain reaction; RT-PCR: Reverse transcription-polymerase chain reaction.