Gelatin degradation assay reveals MMP-9 inhibitors and function of O-glycosylated domain

Figure 3 Enzyme velocity as a function of the amount of substrate (nmol/L DQ™-gelatin) (at a concentration of 1 nmol/L). Prism 5 (GraphPad Software, Inc) was used to fit the data with the corresponding Michaelis-Menten curve and to calculate the V_max and K_M values (Table 2). By using a Wilcoxon signed rank test we determined that all mutants had a significantly different activity from that of matrix metalloproteinase (MMP)-9 FL (^aP < 0.05, ^bP < 0.01). The graphs are representative of three independent experiments.