Soluble and insoluble signals sculpt osteogenesis in angiogenesis

Figure 7 Prominent induction of bone formation by binary applications of recombinant hOP-1 with relatively low doses of recombinant hTGF-β₁ implanted in the rectus abdominis muscle of P. ursinus. A, B: Morphology of tissue induction and rapid tissue growth by binary application of 25 μg hOP-1 and 0.5 μg hTGF-β₁ harvested on day 15 after implantation in the rectus abdominis muscle. A: Induction of a corticalized ossicle on day 15 after the synergistic binary implantation in the rectus abdominis muscle; B: Trabeculae of mineralized bone in blue on day 15 are surfaced by osteoid seams (red-orange) populated by contiguous osteoblasts; C, D: Prominent induction of heterotopic bone after implantation of 25 μg hOP-1 contra laterally juxtaposed to doses of 5 μg[68] across the rectus abdominis muscle of adult non-human primates of the species P. ursinus[68]. The radius of activity of the singly implanted hTGF-β₁ osteogenic devices synergistically enhanced the inductive activity of the contra laterally implanted 25 μg hOP-1 devices[68]; E, F: Large heterotopic mineralized and corticalized ossicles generated by binary application of 25 μg hOP-1 and 0.5 μg hTGF-β₁ and harvested on day 30. Undecalcified sections cut at 6 μm stained free-floating with a modified Goldner’s trichrome.