Shenzhuo formulation ameliorates diabetic nephropathy by regulating cytochrome P450-mediated arachidonic acid metabolism

Figure 4 Effect of Shenzhuo formulation intervention on cytochrome P450-mediated AA metabolism in diabetic nephropathy mice. A: Heatmap of cytochrome P450 (CYP450) metabolism-related gene expression showing that Shenzhuo formulation high dose group (SZFH) intervention significantly up-regulated Cyp2j13, Cyp2b9, Pla2g2e, Cbr3, and Cbr1 mRNA expression and down-regulated Cyp4a12a, Cyp4a32, Cyp2e1, Cyp4a14, and Gm11771 mRNA expression; B: Heatmap of AA metabolism-related product expression showing that SZFH intervened to up-regulated 5,6-EET, 14,15-EET, and Phosphatidylcholine expression and down-regulated 20-HETE expression; C: Translational relationship diagram of CYP450-mediated AA metabolism; D and E: Western blot results showing that CYP4A and CYP2E expression were significantly down-regulated and CYP2J and CYP2B expression were significantly up-regulated after PMS intervention (Supplementary Figure 1). n = 3 for A; n = 6 for B; n = 3 for D and E. ^bP < 0.01 vs control group; ^cP < 0.05; ^dP < 0.01 vs model group; AA: Arachidonic acid; Control: Control group; Model: Model group; CYP450: Cytochrome P450. The original image of Figure 4D is in the Supplementary materials.