Vanadium-dependent activation of glucose transport in adipocytes by catecholamines is not mediated via adrenoceptor stimulation or monoamine oxidase activity

Figure 3 Inhibition by pargyline, semicarbazide, and their combination on amine-induced hexose uptake in rat adipocytes in the presence of 100 µmol/L sodium orthovanadate. Immediately prior to the 10-min 2-deoxyglucose (2-DG) uptake assay, rat fat cells were incubated for 45 min as in Figure 2 in the presence of 100 µmol/L vanadate (orange arrow) without (basal) and with 100 nmol/L insulin (light grey columns), or with the indicated agents (control, black columns). 1 mmol/L pargyline or 1 mmol/L semicarbazide (grey columns) and their combination (open columns) were added to each agent (hydrogen peroxide or amine) tested at 1 mmol/L, except benzylamine, which was tested at 0.1 mmol/L. A significant reduction of 2-DG uptake when compared to the respective control was observed at: ^aP < 0.02. The mean ± SE of 4 adipocyte preparations was determined. 2-DG: 2-Deoxyglucose.