Reversal of multidrug resistance of hepatocellular carcinoma cells by metformin through inhibiting NF-κB gene transcription

Figure 3 Metformin down-regulates P-glycoprotein expression via the nuclear factor-κB signaling pathway. A: After HepG2/ADM cells were treated with different doses of metformin for 24 h, the levels of P-gp and p-p65 expression analyzed by Western blot were decreased in a dose-dependent manner, and the cytoplasma p65 increased in a dose-dependent manner; B: The gray intensity images of Figure 3A. ^aP < 0.05, ^bP < 0.01 vs the blank group (n = 3, mean ± SD); C: After HepG2/ADM cells were treated with 1 mmol/L metformin for different time periods, the levels of P-gp and p-p65 expression analyzed by Western blot were decreased in a time-dependent manner, and the cytoplasma p65 increased in a time-dependent manner; D: The gray intensity images of Figure 3C. ^aP < 0.05, ^bP < 0.01 vs the blank group (n = 3, mean ± SD). P-gp: P-glycoprotein; NF-κB: Nuclear factor-κB; ADM: Adriamycin.