Multistep carcinogenesis of perihilar cholangiocarcinoma arising in the intrahepatic large bile ducts

Figure 5 Semiquantitative evaluation of P16INK4a and EZH2 in BilIN-1, -2, and -3, and invasive cholangiocarcinoma (CC). The labeling index (LI) reflects the percentage of cells immunohistochemically positive for P16^INK4a or EZH2 in each lesion of BilIN-1, -2, and -3, and invasive CC. ^bP < 0.01 vs BilIN-1. A: The LI of P16^INK4a expression (mean ± SD) decreases with the progressing of BilINs and is higher in invasive CC; B: Expression of EZH2 increases with the progression of BilINs and is greater in invasive CC[22].