Role of nanotopography in the development of tissue engineered 3D organs and tissues using mesenchymal stem cells

Figure 8 Electron microscopy images of differentiated and undifferentiated mesenchymal stem cells on Coll/PLLCL nanostructured nanofibrers. A: Mesenchymal stem cell (MSC) directed to differentiate along the epidermal lineage when cultured in epidermal induction medium; B: Epidermally differentiated MSC on the edge of a Coll/PLLCL scaffold (as shown by arrow); C: Electrospun Coll/PLLCL nanofibers seeded with undifferentiated MSC cultured in normal growth medium; D: MSC grown in normal growth medium on Coll/PLLCL nanofibers stained with Ker 10, after 15 d cell culture, imaged using laser scanning confocal microscope[85].