Alternative splicing: An important mechanism in stem cell biology

Figure 2 Methods for profiling alternative splicing events. The diagram depicts methods used for profiling AS events. The cassette exon AS event is used as an illustration for simplicity. “E1” and “E2” are constitutively spliced exons, and “A” is an alternative exon. Probes designed for specific regions are noted with a “Pr-“ prefix. A: Splicing sensitive microarray for AS profiling. Adopted from[2]. B: RNA sequencing for AS profiling. Percent spliced-in value (PSI) #E1A, #E1E2, #AE2 are the number of reads mapped to the spliced junctions. AS: Alternative splicing.