Neural differentiation from pluripotent stem cells: The role of natural and synthetic extracellular matrix

Figure 1 Oligodendrocyte progenitor cells derived from human pluripotent stem cells. A: Morphology of day 41 Oligodendrocyte progenitor cells (OPCs) derived from cells grown on Matrigel; B: Morphology of day 41 OPCs derived from cells grown on vitronectin-derived synthetic peptide acrylate surface (VN-PAS); scale bar: 200 μm; C and D: Oligodendroglial morphology after OPC maturation; C: Low magnification; D: High magnification, scale bar: 100 μm; E: OPC marker expression; MMM: all the steps of human pluripotent stem cell (hPSC) expansion and differentiation were performed on Matrigel; MVV: hPSC expansion on Matrigel and differentiation on VN-PAS; VVV: All the steps of hPSC expansion and differentiation were performed on VN-PAS. ^aP < 0.05 vs MMM. F: Flow cytometry histograms of OPC markers; PDGFRα: Platelet-derived growth factor receptor alpha. This figure is adapted from Li et al[13].