Epigenetics and chromatin plasticity in embryonic stem cells

Figure 1 Morphology of a mouse embryonic stem cells colonies. A, B: Confocal microscopy of mouse embryonic stem cells (mESC) colonies (line GOWT1) stably expressing GFP-OCT4 was performed using a Leica SPX-5 microscope. GFP fluorescence is shown as green and the cell colonies were additionally visualized in transmission light (gray); C, D: Morphology of human embryonic stem cells (hESC) colonies studied by transmission light; E, F: mESCs (line D3) were differentiated into cardiomyocytes (gray) (E) according to report of Veselá et al[113] and α-actinin morphology (green) was studied using the appropriate antibody. Nuclei were visualized by DAPI staining (blue). Cardiomyocytes were characterized by their specific strip-like morphology of α-actinin. Morphology of mouse ESCs differentiated into neuronal pathway[114] is also showed (F).