Hunting down the dominating subclone of cancer stem cells as a potential new therapeutic target in multiple myeloma: An artificial intelligence perspective

Figure 4 Improved clinical outcomes with microfluidic CD45 depletion (Patient 1). A: Bone marrow smear at the time of initial diagnosis; B: Bone marrow smear after effective treatment (complete remission); C: Flow-cytometry without microfluidic enrichment. Plasma cells (CD38+/CD138+) is only 4.24% and no FISH cytogenetic abnormalities were found (low risk); D: After microfluidic enrichment (center inset) plasma cells (CD38+/CD138+) increased to 36.1%; E: FISH on enriched plasma cells show 17p- (Red: D13S319; Green: P53); F: FISH showed del(13q14) in enriched PC (Red: D13S319; Green: RB1). With enriched plasma cell for FISH, the patient was reclassified and treated as high-risk multiple myeloma which leads to complete remission (Refer to[17] for details).