Effects of DNA methylation on expression of tumor suppressor genes and proto-oncogene in human colon cancer cell lines

Figure 3 5-aza-dC increased the transcription of p16^INK4A gene in Colo-320 (A) and SW1116 cells. Lane 1: mock treatment. Lanes 2-7: after 5-aza-dC treatment; lane 2: 2 μmol/L, 24 h; lane 3: 5 μmol/L, 24 h; lane 4: 10 μmol/L, 24 h; lane 5: 2 μmol/L, 72 h; lane 6: 5 μmol/L, 72h; lane 7: 10 μmol/L, 72 h. The density of bands shown in Table 3.