S-phase delay in human hepatocellular carcinoma cells induced by overexpression of integrin β1

Figure 4 Activation of PKB, but not FAK, was downregulated in β1- and α5β1- transfected cells. (A) FAK activation was as-sessed by phosphotyrosine- specific antibody, followed by stripping and reprobing with anti-FAK antibody. (B) PKB and its Ser473-phosphorylated forms were determined by 10% SDS-PAGE with the equal loading amount. The loading amount control is shown in Figure 1C and Figure 3A. Lane 1, mock cells; lane 2, α5-7721; lane 3, β1-7721; lane 4, α5β1-7721. Results were representative of 4 repeated experiments.