Expression of ECRG4, a novel esophageal cancer-related gene, downregulated by CpG island hypermethylation in human esophageal squamous cell carcinoma

Figure 4 (a) The methylation detection of ssPCR products at 56 °C on DHPLC. The methylation peak was emphasized by the arrow. PC was the product from the SssI treated human placenta genomic DNA. 1, 2, 3, 4; the same samples as Figure 2. (b) The expression level of ECRG 4 by RT-PCR using the primer set flanking the ORF of the gene in the same samples was detected on DHPLC. The β-Actin gene was amplified as internal control. M; 1 kb DNA Ladder Marker. 1, 2, 3, 4; the same samples as Figure 2.