Suberoylanilide hydroxamic acid upregulates reticulophagy receptor expression and promotes cell death in hepatocellular carcinoma cells

Figure 6 The impact of suberoylanilide hydroxamic acid treatment on mitochondrial membrane potential and the expression of proteins related to the mitochondrial apoptotic pathway in Huh7 and MHCC97L cells. A and B: After being treated with 0, 1, 3, 6, and 12 μmol/L suberoylanilide hydroxamic acid for 48 h, the mitochondrial membrane potential in Huh7 and MHCC97L cells was analyzed using flow cytometry with bivariable JC-1 dye (mitochondrial membrane potential probe). Representative pictures from three independent replicate assays are exhibited; C-E: Representative western blot images showed the related protein expression in Huh7 and MHCC97L cells treated with 0, 1, 3, 6, and 12 μmol/L suberoylanilide hydroxamic acid for 48 h. Relative protein expression levels were normalized to β-actin. Data are exhibited as mean ± standard deviation. ^aP < 0.05 vs 0 μmol/L group (n = 3).