Non-exposed endoscopic wall-inversion surgery with one-step nucleic acid amplification for early gastrointestinal tumors: Personal experience and literature review

doi:10.3748/wjg.v29.i24.3883

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Jun 28, 2023 (publication date) through Dec 29, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 28, 2023; 29(24): 3883-3898
Published online Jun 28, 2023. doi: 10.3748/wjg.v29.i24.3883

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Figure 4 One-step nucleic acid amplification assay. A-F: Lymph nodes were prepared and placed in homogenized lysis buffer (Lynorhag; Sysmex) and then centrifuged. CK19 mRNA was extracted from the lysate and analyzed by reverse transcription-loop-mediated isothermal amplification in the RD-100i system (Sysmex) using the Lynoamp (Sysmex) reagent kit[59].

Citation: Crafa F, Vanella S, Morante A, Catalano OA, Pomykala KL, Baiamonte M, Godas M, Antunes A, Costa Pereira J, Giaccaglia V. Non-exposed endoscopic wall-inversion surgery with one-step nucleic acid amplification for early gastrointestinal tumors: Personal experience and literature review. World J Gastroenterol 2023; 29(24): 3883-3898
URL: https://www.wjgnet.com/1007-9327/full/v29/i24/3883.htm
DOI: https://dx.doi.org/10.3748/wjg.v29.i24.3883