Metabolomics of Fuzi-Gancao in CCl4 induced acute liver injury and its regulatory effect on bile acid profile in rats

Table 9 Precision, accuracy, and recovery of bile acids in serum samples (n = 6, mean ± SD)

Compound	Concentration (ng/mL)	Recovery (%)	Intraday		Interday
			Accuracy (%)	Precision (%)	Accuracy (%)	Precision (%)
CA	2.5	90.12 ± 2.16	96.44 ± 2.05	4.26	91.83 ± 3.32	5.03
	500	88.21 ± 5.34	91.65 ± 4.14	3.60	94.42 ± 4.55	4.36
	2000	85.60 ± 7.22	92.11 ± 4.11	5.42	94.54 ± 8.35	6.00
GLCA	1.25	91.52 ± 5.51	92.13 ± 2.97	5.71	98.48 ± 2.22	3.75
	250	86.74 ± 7.12	87.31 ± 1.92	4.23	93.42 ± 3.67	5.40
	1000	90.48 ± 3.31	91.55 ± 5.31	6.13	89.24 ± 4.63	4.57
GDCA	1.25	88.54 ± 2.93	90.67 ± 6.87	6.22	88.55 ± 3.87	3.28
	250	98.43 ± 1.26	97.24 ± 7.86	6.38	101.53 ± 2.54	2.76
	1000	95.56 ± 4.01	89.34 ± 2.65	2.81	89.23 ± 6.25	6.16
GCDCA	1.25	96.02 ± 3.11	93.24 ± 3.78	6.30	89.32 ± 4.32	3.19
	250	90.13 ± 3.42	97.43 ± 2.82	5.03	88.54 ± 4.75	2.10
	1000	89.32 ± 3.13	86.53 ± 4.27	2.96	89.52 ± 3.63	3.28
GCA	1.25	80.87 ± 3.83	99.31 ± 5.19	4.18	89.42 ± 1.71	2.32
	250	91.43 ± 6.38	101.33 ± 2.23	2.59	112.53 ± 5.76	2.29
	1000	92.43 ± 2.14	88.76 ± 1.46	1.20	89.16 ± 3.46	3.09
TCA	2.5	84.77 ± 2.38	103.55 ± 1.27	1.50	98.24 ± 2.14	5.51
	500	86.92 ± 4.96	89.04 ± 6.42	5.53	95.33 ± 2.86	3.82
	2000	81.34 ± 1.84	88.57 ± 4.85	4.77	97.81 ± 3.08	4.50
CDCA	1.25	80.33 ± 3.23	94.33 ± 4.62	4.39	90.49 ± 4.83	5.18
	250	86.34 ± 2.45	92.33 ± 4.93	3.70	91.49 ± 7.39	4.49
	1000	84.62 ± 3.21	92.33 ± 6.03	5.58	92.77 ± 4.83	6.18
DCA	1.25	95.54 ± 2.17	94.67 ± 4.93	5.88	104.21 ± 7.22	3.87
	250	82.56 ± 4.32	106.33 ± 7.37	4.36	99.31 ± 5.66	5.57
	1000	94.45 ± 5.34	101.33 ± 5.77	6.31	89.83 ± 4.53	4.71
UDCA	1.25	83.32 ± 1.43	91.67 ± 4.62	6.41	93.43 ± 4.63	3.38
	250	88.13 ± 5.94	95.33 ± 4.73	6.57	92.12 ± 4.49	2.84
	1000	85.26 ± 3.01	94 ± 4.58	2.90	91.79 ± 5.74	6.35
TCDCA	1.25	86.04 ± 3.36	93.67 ± 5.86	6.49	92.77 ± 4.83	3.28
	250	87.98 ± 3.11	94.67 ± 4.93	5.18	89.83 ± 4.53	2.17
	1000	83.72 ± 2.19	91.67 ± 4.62	3.05	92.77 ± 7.36	3.38
TUDCA	1.25	87.17 ± 4.61	94.67 ± 7.51	4.31	96.69 ± 9.82	2.39
	250	87.41 ± 4.09	98.67 ± 10.02	2.66	98.33 ± 8.33	2.36
	1000	99.06 ± 3.56	100.33 ± 8.5	1.23	92.45 ± 4.53	3.18

CDCA: Chenodeoxycholic acid; UDCA: Ursodeoxycholic acid; GCA: Glycocholic acid; GDCA: Glycochenodeoxycholic acid; TCA: Taurocholic acid; TUDCA: Taurocholic acid; GCDCA: Glycochenodeoxycholic acid; GLCA: Glycinic acid; DCA: Deoxycholic acid; CA: Cholic acid.