Published online Oct 28, 2021. doi: 10.3748/wjg.v27.i40.6888
Peer-review started: April 17, 2021
First decision: June 23, 2021
Revised: June 28, 2021
Accepted: August 25, 2021
Article in press: August 25, 2021
Published online: October 28, 2021
Fuzi (Radix aconiti lateralis)-Gancao (Radix glycyrrhizae) (F-G) is often used in the treatment of liver diseases such as hepatitis and liver failure.
This study can clarify the bile acid mechanism of F-G in the treatment of liver injury, and establish a complete bile acid spectrum research method, so as to provide reference for future research.
To study the molecular mechanism and action mechanism of F-G in the treatment of liver injury, and to provide a theoretical basis for the clinical research of F-G.
An ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of 92 metabolites from multiple pathways was established to explore the protective metabolic mechanism of F-G in serum on the liver.
A UPLC-MS/MS method for simultaneous determination of 11 bile acids was established to analyze the regulatory mechanism of F-G in serum. F-G decreased the contents of 11 bile acids in the serum in a dose-dependent manner.
F-G could promote the conjugation of free bile acids to glycine and taurine, reduce the accumulation of free bile acids in the liver, regulate the compensatory degree of taurine, and decrease the content of taurine conjugated bile acids.
The research group will continue to study the effect of bile acid metabolism regulation on molecular regulation in the body.