Metabolomics of Fuzi-Gancao in CCl4 induced acute liver injury and its regulatory effect on bile acid profile in rats

Table 3 Accuracy and precision of the developed ultra performance liquid chromatography-tandem mass spectrometry method (n = 6)

Ingredient	Concentration (ng/mL)	Accuracy %	Precision %	Repeatability %	Stability %
L-Leu	750	100.35 ± 3.20	3.19	3.69	0.41
	1000	105.20 ± 6.45	6.13	6.91	1.69
	5000	95.53 ± 4.55	4.76	4.98	1.85
5-HT	6	99.78 ± 8.61	8.63	8.10	2.78
	8	101.78 ± 4.32	4.24	2.87	3.80
	40	101.76 ± 4.50	4.42	4.24	2.14
N-phe	1800	100.29 ± 4.32	4.31	4.72	3.07
	2500	98.97 ± 4.13	4.17	3.71	3.18
	12500	92.24 ± 5.12	5.55	5.95	1.09
L-Try	4500	97.33 ± 3.25	3.34	3.75	0.71
	6000	95.45 ± 4.12	4.32	4.22	3.35
	30000	94.89 ± 4.77	5.03	4.22	4.00
L-Kyn	37	99.61 ± 4.66	4.68	5.00	2.98
	50	102.43 ± 4.15	4.05	4.41	1.73
	250	99.76 ± 4.11	4.12	4.15	2.09
5-HTP	300	102.45 ± 4.51	4.40	3.98	3.65
	400	95.20 ± 8.80	9.24	7.50	6.16
	2000	101.77 ± 8.11	7.97	7.89	7.36
CA	1.5	99.88 ± 3.70	3.70	3.83	1.47
	2	97.00 ± 3.19	3.29	2.97	2.47
	10	96.76 ± 5.30	5.48	4.17	5.22
GSH	1500	101.91 ± 4.65	4.56	5.19	1.45
	2000	96.05 ± 3.98	4.14	4.63	0.89
	10000	97.51 ± 5.88	6.03	4.45	2.45
GSSG	750	99.64 ± 3.97	3.98	4.43	1.56
	1000	100.8 ± 6.45	6.40	7.23	4.89
	5000	98.39 ± 4.54	4.61	3.90	3.80

L-Leu: L-leucine; 5-HT: 5-Hydroxytryptamine; N-phe: N-phenylacetylglycine; L-Try: L-tryptophan; L-Kyn: L-kynurenine; 5-HTP: 5-hydroxytryptophan; CA: Cholic acid; GSH: Glutathione; GSSG: Glutathione oxidized.