Metabolomics of Fuzi-Gancao in CCl4 induced acute liver injury and its regulatory effect on bile acid profile in rats

Table 2 Calibration curve, R², and concentration range of nine metabolites

Compound	Standard curve	R2	Range (ng/mL)
L-Leu	Y = 0.0627X - 4.9797	0.9952	100-10000
5-HT	Y = 0.0385X - 0.0269	0.9939	0.8-80
N-phe	Y = 0.0229X - 4.9120	0.9958	250-25000
L-Try	Y = 0.0048X - 2.4673	0.9926	600-60000
L-Kyn	Y = 0.0331X + 0.0123	0.9960	5-500
5-HTP	Y = 0.0492X - 0.0001	0.9983	0.2-20
CA	Y = 0.00004X - 0.0010	0.9959	40-4000
GSH	Y = 0.0032X - 0.2362	0.9975	150-20000
GSSG	Y = 0.0001X + 0.0010	0.9949	50-10000

L-Leu: L-leucine; 5-HT: 5-Hydroxytryptamine; N-phe: N-phenylacetylglycine; L-Try: L-tryptophan; L-Kyn: L-kynurenine; 5-HTP: 5-hydroxytryptophan; CA: Cholic acid; GSH: Glutathione; GSSG: Glutathione oxidized.