Metabolomics of Fuzi-Gancao in CCl4 induced acute liver injury and its regulatory effect on bile acid profile in rats

Table 10 Stability of bile acid standards in serum samples (n = 6, mean ± SD)

Compound	Concentration (ng/mL)	Concentration (mean ± SD)
		Initial	Freeze-thaw	Short-term	Long-term	Post-preparation
CA	2.5	2.35 ± 0.17	2.3 ± 0.16	2.56 ± 0.18	2.33 ± 0.17	2.52 ± 0.08
	500	495.43 ± 12.15	485.52 ± 11.91	538.93 ± 13.22	511.98 ± 12.56	505.09 ± 5.54
	2000	2080.38 ± 101	2038.77 ± 98.98	2263.03 ± 109.87	2149.88 ± 104.38	2194.52 ± 99.16
GLCA	1.25	1.24 ± 0.14	1.22 ± 0.13	1.17 ± 0.15	1.21 ± 0.14	1.25 ± 0.14
	250	247.1 ± 10.76	242.15 ± 10.55	258.79 ± 11.71	255.35 ± 11.12	250.24 ± 10.9
	1000	979.04 ± 40.32	959.46 ± 39.51	985 ± 43.86	1011.75 ± 41.66	991.52 ± 40.83
GDCA	1.25	1.27 ± 0.05	1.24 ± 0.05	1.24 ± 0.06	1.19 ± 0.06	1.26 ± 0.08
	250	240.43 ± 3.98	235.62 ± 3.9	261.54 ± 4.33	248.46 ± 4.12	249.32 ± 5.42
	1000	975.71 ± 45.93	956.19 ± 45.01	1061.38 ± 49.96	1008.31 ± 47.46	1035.03 ± 14.83
GCDCA	1.25	1.27 ± 0.06	1.23 ± 0.08	1.36 ± 0.08	1.21 ± 0.09	1.21 ± 0.08
	250	243.49 ± 4.03	242.1 ± 19.01	237.25 ± 18.63	263.35 ± 20.68	250.18 ± 19.64
	1000	988.14 ± 46.51	1047.04 ± 49.29	1026.1 ± 48.31	1108.97 ± 53.62	1082.02 ± 50.94
GCA	1.25	1.19 ± 0.03	1.27 ± 0.03	1.27 ± 0.03	1.22 ± 0.03	1.21 ± 0.04
	250	237.1 ± 1.93	232.35 ± 1.9	257.91 ± 2.1	245.02 ± 2	244.16 ± 1.88
	1000	982.38 ± 30.18	962.73 ± 29.58	1068.63 ± 32.83	1015.2 ± 31.19	1014.36 ± 44.06
TCA	2.5	2.25 ± 0.17	2.21 ± 0.16	2.45 ± 0.18	2.33 ± 0.17	2.42 ± 0.08
	500	492.1 ± 28.22	482.25 ± 27.65	535.3 ± 30.69	508.54 ± 29.16	494.76 ± 23.69
	2000	2013.71 ± 105.21	1973.43 ± 103.1	2190.51 ± 114.44	2080.99 ± 108.72	2142.85 ± 26.09
CDCA	1.25	1.19 ± 0.1	1.28 ± 0.1	1.23 ± 0.11	1.24 ± 0.1	1.22 ± 0.08
	250	232.1 ± 6.9	227.45 ± 6.77	252.47 ± 7.51	239.85 ± 7.14	236.41 ± 5.54
	1000	1010.38 ± 39.44	990.17 ± 38.65	1099.09 ± 42.91	1044.13 ± 40.76	1057.77 ± 46.98
DCA	1.25	1.13 ± 0.03	1.15 ± 0.06	1.18 ± 0.13	1.23 ± 0.13	1.24 ± 0.14
	250	236.33 ± 13.65	229.67 ± 21.36	233 ± 10.15	244.65 ± 10.66	247.1 ± 10.76
	1000	967.67 ± 15.82	947.67 ± 106.82	1031 ± 115.66	982.55 ± 53.41	979.04 ± 40.32
UDCA	1.25	1.22 ± 0.06	1.19 ± 0.06	1.23 ± 0.07	1.26 ± 0.07	1.28 ± 0.07
	250	252.1 ± 6.9	247.05 ± 6.77	244.23 ± 7.51	250.52 ± 7.14	257.07 ± 5.54
	1000	931.71 ± 85.92	971.43 ± 120.15	958.15 ± 108.46	940.89 ± 109.04	1061.11 ± 99.16
TCDCA	1.25	1.22 ± 0.06	1.25 ± 0.06	1.28 ± 0.07	1.22 ± 0.06	1.19 ± 0.06
	250	251.76 ± 3.11	246.73 ± 3.05	273.87 ± 3.39	260.17 ± 3.22	261.73 ± 2.5
	1000	1013.71 ± 69.97	993.43 ± 68.57	1102.71 ± 76.11	1047.58 ± 72.31	1057.77 ± 99.16
TUDCA	1.25	1.22 ± 0.02	1.2 ± 0.02	1.23 ± 0.03	1.26 ± 0.02	1.27 ± 0.03
	250	249.1 ± 6.47	244.11 ± 6.34	270.97 ± 7.04	257.42 ± 6.68	259.14 ± 8.46
	1000	977.04 ± 18.84	957.5 ± 18.46	1062.83 ± 20.49	1009.69 ± 19.47	1006.1 ± 26.09

CDCA: Chenodeoxycholic acid; UDCA: Ursodeoxycholic acid; GCA: Glycocholic acid; GDCA: Glycochenodeoxycholic acid; TCA: Taurocholic acid; TUDCA: Taurocholic acid; GCDCA: Glycochenodeoxycholic acid; GLCA: Glycinic acid; DCA: Deoxycholic acid; CA: Cholic acid.