Aberrant methylation of secreted protein acidic and rich in cysteine gene and its significance in gastric cancer

Figure 3 Hypermethylation status of secreted protein acidic and rich in cysteine promoter in gastric cancer cells and the effects of drug intervention. A: Representative statistics of methylation-specific polymerase chain reaction (PCR) in five cell lines. PCR visualized in 2% agarose gels; B: Relative SPARC mRNA expression levels of BGC-823 cells after drug intervention were analyzed by quantitative real-time PCR followed by quantitation relative to glyceraldehyde-3-phosphate dehydrogenase; C: Methylation status of promoter region after drug intervention. Data are expressed as mean ± standard deviation (Figure 3B). ^aP < 0.05 was considered statistically significant. SPARC: Secreted protein acidic and rich in cysteine; M: Methylated amplified fragment; U: Unmethylated amplified fragment; MP: Methylated positive control; UP: Unmethylated positive control; ddH₂O: Blank control.