Human bocavirus: Current knowledge and future challenges

Figure 2 Similarity plot (generated by SimPlot)[56] of Human Bocaviruses genomes. Each curve is a comparison between the HBoV1 genome (reference) and HBoV2-4 sequences. Nucleotide sequences were aligned using Clustal Omega[57] and the plot was rendered by Simplot using a window size of 200 bp and a step size of 20 bp. The horizontal bars above the curves represent the HBoV1 genes arranged as indicated in Figure 1; Phylogenetic trees of nucleotide and amino acid sequences of the HBoV genes. Sequences were aligned by Clustal Omega and phylogenetic analysis was carried out using the Neighbor-Joining method implemented in the MEGA5 program[58]. The numbers at the branch nodes indicate the bootstrap values calculated with 1000 replicates.